Purificación en gran escala de una variedad del virus del mosaico del tabaco hallada en Cuba

Authors

  • S. QUINTERO Red Nac. de Investigaciones Tabacaleras, MINAG
  • S. KORNEVA Red Nac. de Investigaciones Tabacaleras, MINAG
  • R. H. MARIBONA Centro Nacional de Investigaciones Científicas

Abstract

A Tobacco Mosaic Virus st.rain (TMVsc) is purified by mean of the ultraccntrifugation in a sucrose grádient, in a titanium zonal rotor B-XV, in a continuous flow rotor, by different.ial centri­fugation and by Sephadex G-200. Different buffers, ionic strengh, and times are used for the dialysis. 500 mg of pure, active, and nonaggre­gated virus, analyzed by the ultraccntrifugation and the analytical sucrose gradient, were purified.

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Published

2022-07-19

How to Cite

QUINTERO, S. ., KORNEVA, S., & MARIBONA, R. H. (2022). Purificación en gran escala de una variedad del virus del mosaico del tabaco hallada en Cuba . NATIONAL CENTER FOR SCIENTIFIC RESEARCH (CENIC) BIOLOGICAL SCIENCES JOURNAL, 11(1), 163-175. Retrieved from https://revista.cnic.cu/index.php/RevBiol/article/view/1698

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Section

Research articles