OBTENCION DE ANTICUERPOS POLICLONALES CONTRA PROTEINAS GLICOSILADAS NO ENZIMATICAMENTE

Authors

  • J. López Ruíz Instituto Nacional de Angiología y Cirugía Vascular
  • B. Gabrysiak Instituto Nacional de Angiología y Cirugía Vascular
  • L. Sorell Gómez Instituto Nacional de Angiología y Cirugía Vascular

Abstract

A procedure for the obtainment of specific antibod­ies against non- enzymatically glycosylated proteins is describ­ed.Balb/c mouses were inmunized with homologous serum glycosylated in vitro with the addition of glucose and a reducing agent. The specificity of the antiserum extracted from the inmu­nized mouses was tested in a solid phase inmunoassay (ELISA) in which it was able to recognize glycosylated proteins from different origins, while these same proteins in native state were not recog­nized.

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Published

2022-11-24

How to Cite

López Ruíz, J. ., Gabrysiak, B. ., & Sorell Gómez, L. . (2022). OBTENCION DE ANTICUERPOS POLICLONALES CONTRA PROTEINAS GLICOSILADAS NO ENZIMATICAMENTE . NATIONAL CENTER FOR SCIENTIFIC RESEARCH (CENIC) BIOLOGICAL SCIENCES JOURNAL, 24(1-2-3), 015-017. Retrieved from https://revista.cnic.cu/index.php/RevBiol/article/view/3134

Issue

Section

Research articles