Métodos de disolución en muestras de hidroxiapatita y corales

Abstract

The utilization of samples of hidroxiapatite and corals in implant bone requires to know the content of sorne metals with accuracy. High contents of Cu and Pb could exclude the use of these samples with this purpose. This pa­per was studied three methods of preparation of samples (conventional diges­tion, use of microwave in closed containers and preparation of suspensions) for
the determination ofthe Cu and Pb in samples ofhidroxiapatite [Ca10(P04)/0H)z] and corals for Atomic Absorption Spectrometry with Electrothermal Atomization, AAS-EA and correction by Zeeman effect. The advantages of the process of disolution with microwave and the preparation of suspensions in relationship to the conventional digestion were checked with respect to saving of reagents and time and the least possibi lity of contamination. Fbr the determinations of metals, the parameters of operation of the spectrometer were optimized, as well as the heating program of the oven for each element. An international reference mate­rial was utilized (IAEA-H-5 Bone Animal) in order to check the accuracy of the determinations of Pb. In case of the determinations of Cu tests of recuperation were carried out getting security values between 95-105 %. The detection limits were calculated according to the IUPAC criterion and the results were: 0,15 and 1,05 ng · mL'1for Cu and Pb respectively. The levels ofthese metals found in the samples are low and permit the utilization of the same in implant bones.