Recuperación de columnas de extracción en fase sólida DetectabuseTM para la detección de esteroides anabólicos en el control doping

Abstract

DetectabuseTM extraction columns have been developed for the
quantitative extraction of several families of drugs from biological fluids and
due to their versatility and robustness they are, at present time, widely used in
solid phase extraction procedures (SPE). One of their advantages is the ability
to extract, in an efficient way, a wide range of compounds of the most common
drugs of abuse, drugs of therapeutic activity and steroids excreted in free and
conjugated form in urine. DetectabuseTM columns are used in doping for sample
preparation in the analysis of anabolic steroids and other compounds excreted
in urine and have, as main objective, to remove the inorganic salts excreted in
the urine and to regulate the efficiency of the enzymatic hydrolysis process.
This paper shows the results of the regeneration of these columns for their later
use in screening procedures. The study was carried out with the following compounds:
methenolone, methenolone metabolite, boldenone, boldenone metabolite,
bolasterone metabolite, fluoxymesterone, drostanolone metabolite,
mesterolone metabolite, methandienone metabolite1, danazol metabolite,