Aplicación de las cartas simples de control en el laboratorio antidoping

Abstract

Control charts are the methods used in any assay laboratory to carry
out the internal control of the analytic processing of samples. They are graphic
representations of data according to certain construction rules that allow, among
others, to demonstrate the statistical control state of the analytical system, to
carry out the pursuit of a measuring process, to document critical aspects of the
sampling and having a control of the reproducibility and repeatability of the analysis.
The present paper shows, in a simple way, the application of control charts,
specifically the graphs of Shewhart in the internal quality control system of the
antidoping laboratory. Beginning from the observation of an extraction yield decreasing
or the recovery of the androsterone in the usual extractive procedure for
the detection of anabolic steroids and other compounds, a series of assays were
carried out to analyze the analysis of causes. The results indicated a loss of the
activity of the enzyme ß-glucuronidase used in the extractive procedure. The
tests guided in this sense included the use of an etiocholanolone-glucuronide
solution, where data were referred to the appearance of the substrate free of the
group glucuronide. In second place a reference enzyme with the same characteristics
was employed. Data obtained in the study confirmed the loss of the activity
of the enzyme β-glucuronidase (E. coli) used in the detection of anabolic steroids
and other compounds in the laboratory.