Caracterización morfométrica de una línea celular de la caña de azúcar

Abstract

Conventionally, the morphological control of cell suspensions is performed by means of microscopic observations and the measurements are to carry out by means of graticules or photographies, but this method spend much time and offers limited exactness. To test the stabilization of cell line a method is described for the morphometric analysis by means of image processing of the morphological changes in cell suspension (Var. C87-51) of sugarcane. Perimeter, diameter, compactibility, area, size, specific rate of growth and electrophoretical pattern of peroxidase enzimes in the second-seventh subcultures were determined. Perimeter and diameter of the cells were the better variables for morphometrical characterization of cell suspension. These variables explained
73 .2 % of the variation existing into the sample, as Principal Component Analysis. It demostrated that the celullar line stabilized it's morphological characteristics, beggining with the four subculture with a predominance of spherical cells of a less diameter and perimeter. The growht of cell suspensions permited to know the convenient time to take a sample of celullar suspension. The slope curve of cellular growth in the exponential phase stabilizes beginning with the fourth subculture being its great value in the control suspension. Variations of electrophoretic behaviour of peroxidases in the three sooner subcultures were observed, and stabilized beginning with the four subculture. The growth of cell suspensions so electrophoretic behaviour of peroxidases of the different subcultures, corroborate the morphological findings during stabilization of the cell line.