Efecto de un extracto hipoglicemiante de la Petiveria Alliacea L. sobre la unión de la insulina a eritrocitos

Abstract

The effect of a hypoglycemic extract of Petiveria alliacea (sheilina) on the binding of insulin to erythrocytes of healthy voluntary
donors is studied. Erythrocytes (1,8 · 10⁹ cells) were incubated with 50 pg of monoyodoinsulin, growing concentrations of the non-marked ligand (0; 0,13; 1,38; 4,2; 12,6; 37,2; 111; 333 and 1 000 ng/mL) and 50 mL of a 1 UD/10 mL solution of Petiveria alliacea L.* To determine if the extract had a dosesresponse effect the erythrocytes, monoyodoinsulin and growing doses of the hypoglycemic product (0,005; 0,01; 0,5 and 5 UD/mL) were incubated. In the displacement curve there was a deviation to the right of the ED-50 in
the presence of the extract. To displace 50 % of the bound monoyodoinsulin more than twice the amount of cold insulin was necessary. There was an increment of the specific binding of insulin to the binding sites of the cell surface for all the explored doses of product. A dose-response behavior with a tendency to system saturation was found. These results suggest that the hypoglycemic effect of the extract is linked to insulin action, and probably exerts a reducing action on the erythrocyte's membrane which increases the number of binding sites of insulin to the cell membrane.