Purificaci6n de la enzima lactato deshidrogenasa (H4) de corazón humano

Authors

  • E. PENTÓN ARIAS
  • R. GONZALEZ SUAREZ Centro Nacional de Investigaciones Cientificas

Abstract

Human heart (H,) LdH can be purified from a crude homogenate in 0.02 M TRIS (HCL) buffer pH 7 .0 with EDTA 0 .001 M and mercapto ethanol 0 .007 M, by a one step cromatographic procedure in a 2.5 X 40 cm column of DEAF-Sephadex A-50 swollen and equilibrated in the same buffer. A single peak of enzymatic activity is eluted with a 0 - 0 .3 M NaCl linear gradient in 2 liters total effluent volume at the approximate concentration of 0 .19 M NaCl .

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Published

2022-11-18

How to Cite

PENTÓN ARIAS, E. ., & GONZALEZ SUAREZ, R. . (2022). Purificaci6n de la enzima lactato deshidrogenasa (H4) de corazón humano. NATIONAL CENTER FOR SCIENTIFIC RESEARCH (CENIC) BIOLOGICAL SCIENCES JOURNAL, 5(1), 095-098. Retrieved from https://revista.cnic.cu/index.php/RevBiol/article/view/2954

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Section

Research articles