Evaluación de la irritabilidad dérmica y oftálmica de la formulación de aceite de girasol ozonizado en conejos

Autores/as

  • Zullyt Zamora Rodríguez Departamento de Farmacología-Toxicología Experimental. Unidad de Productos Naturales, CNIC, La Habana, Cuba
  • Ariadne Gutierrez Martínez Departamento de Farmacología-Toxicología Experimental. Unidad de Productos Naturales, CNIC, La Habana, Cuba
  • Leyanis Ocaña Napoles Departamento de Farmacología-Toxicología Experimental. Unidad de Productos Naturales, CNIC, La Habana, Cuba
  • Daniela Urquiaga Riso Departamento de Farmacología-Toxicología Experimental. Unidad de Productos Naturales, CNIC, La Habana, Cuba
  • Oscar Ledea lozano Departamento de Química analítica y Formulaciones. Unidad de Productos Ozonizados. CNIC, La Habana, Cuba
  • Lidia Asela Fernández García Departamento de Química analítica y Formulaciones. Unidad de Productos Ozonizados. CNIC, La Habana, Cuba
  • Dayana Gil Ibarra Departamento de Química analítica y Formulaciones. Unidad de Productos Ozonizados. CNIC, La Habana, Cuba

Resumen

Ozonized vegetable oils and their formulations are used, for their beneficial effects, however, it is important to demonstrate their safety. For this reason, the evaluation of its possible dermal and ophthalmic irritant effect in rabbits of the formulation based on ozonized sunflower oil (AGO) at 30% was performed. for this purpose, New Zealand male young adult rabbits of 1.8 to 2.2 kg of body weight were used, obtained from the National Center for Laboratory Animals (CENPALAB). A group of three animals was used for dermal irritability and another group of equal number for ophthalmic irritability. For dermal irritability, 0.5 g of the AGO cream was applied to an area of ​​previously shaved skin, and covered with a patch. The patch was removed after the first hour to observe the evolution of the degree of edema and erythema according to the Draize scale at 1, 24, 48 and 72 h. For ophthalmic irritability, 0.1g of the 30% AGO cream was applied in the right conjunctival sac of each animal. The evaluation of the degree of affectation for each of the ocular structures, were performed at 1, 24, 48 and 72 h after application. Slight irritation was observed in the first 24 h, therefore, it was classified as mildly irritating. Regarding ophthalmic irritability, there was a slight presence of edema and injection of the conjunctival blood vessels grade 1 and 2 that reverts between 48 and 72 h later, being classified as non-irritating. The 30% AGO cream was classified as mild dermal irritant and non-ophthalmic irritant

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Publicado

2019-12-11

Cómo citar

Zamora Rodríguez, Z., Gutierrez Martínez, A., Ocaña Napoles, L., Urquiaga Riso, D., Ledea lozano, O., Fernández García, L. A., & Gil Ibarra, D. (2019). Evaluación de la irritabilidad dérmica y oftálmica de la formulación de aceite de girasol ozonizado en conejos. Revista CENIC Ciencias Biológicas, 50(3), 242-253. Recuperado a partir de https://revista.cnic.cu/index.php/RevBiol/article/view/311

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