Obtención de anti IgG humana en aves

Abstract

In the current decade eggs yolks from immunized hens have been recognized as an excellent source of polycional antibodies. For this reason it was considered advantageous to obtain an (IgY) anti- human IgG in egg yolk to be used in immunoprecipitation methods. Twelve Leghorns hens of 25 weeks of age were used as animal model. The antigen used was intact human IgG (INTACGLOBIN°) with complete and incomplete adjuvant of Freund (SIGMA Co . Ltd .). The dose was of 100 ±g/animal. Seven
inoculations were carried out every seven day. The dynamic of production of antibodies was determined by immunoprecipitation methods in serum and yolk. Method of precipitation of lipids with sterile water was developed and concentration with PEG 6000, the purity level of the delipidate was determined by SDS-PAGE. The activity of delipidation was evaluated by Immunoelectrophoresis to human IgG. These results suggest that the immunization of hens with human IgG could be used in precipitation methods as Radial single immunodiffusion and routine Immunoelectrophoresis at Clinical Immunology Laboratories, the use of this technology could
reduce the productive cost of antibodies for the conventional methods from sera of mammals at least up to 10 times, besides being a compatible technology with the standards of ICLAS for protection of animals biomodels.